Lifaha tsa makenete li sebelisoa haholo tlhahlobong ea 'mele ea ho itšireletsa mafung, tlhahlobo ea limolek'hule, tlhoekiso ea protheine, ho hlopha lisele le mafapha a mang.
Immunodiagnosis: Lifaha tsa Immunomagnetic li entsoe ka likaroloana tsa makenete le lisebelisoa tse nang le lihlopha tse sebetsang tse sebetsang. Li-protein ligands (li-antigen kapa li-antibodies) li kopantsoe ka thata le lihlopha tse sebetsang tsa lifaha tsa makenete, 'me joale immunoassay e etsoa ho sebelisoa liprotheine tsa lifaha tsa makenete.
Tlhahlobo ea limolek'hule (nucleic acid extract): Lifaha tsa makenete tsa Nanoscale tse nang le lihlopha tse ka holim'a metsi tse ka khonang ho hlahisa nucleic acid li ka aroloa 'me tsa khabisoa ka matla a khoheli,' me tsa e-ba teng ho fumana template ea nucleic acid.
Ho hloekisoa ha liprotheine: Agarose e hokahaneng ka kopanelo hammoho le protheine e kopaneng ea A/G holim'a lifaha tsa makenete, protheine e tlamang ea ProteinA/G, 'me qetellong ea lokoloha ho fumana li-antibodies tse hloekisitsoeng.
Tlhahlobo ea Immune le Tlhahlobo ea Limolek'hule:
E 'ngoe ea ts'ebeliso ea mantlha ea lifaha tsa makenete ke tlhahlobo ea 'mele ea ho itšireletsa mafung, moo e fetohileng lisebelisoa tsa bohlokoa bakeng sa ho lemoha mafu ka nepo. Tšobotsi e ikhethang ea lifaha tsa makenete e hlaha ka bokhoni ba bona ba ho hapa le ho arola li-antigen kapa li-antibodies tse itseng ho lisampole tsa mokuli, ho nolofatsa mokhoa oa ho hlahloba. Ka ho hokahanya li-protein ligands, joalo ka li-antigen kapa li-antibodies, ho lihlopha tse sebetsang tsa lifaha tsa makenete, bafuputsi ba ka etsa liteko tsa ho itšireletsa mafung ka nepo le ka nepo e ntlafalitsoeng.Thuto e 'ngoe e hlollang ea ho hlahloba limolek'hule, e rua molemo o moholo tšebelisong ea lifaha tsa makenete. Ha mekhoa ea tlhahlobo ea limolek'hule e ntse e hlahella lilemong tsa morao tjena, lifaha tsa makenete li bapala karolo ea bohlokoa ho aroleng le ho ntša li-nucleic acid, tse kang DNA kapa RNA, ho tsoa lisampong tsa baeloji. Lifaha tsena li sebetsa e le litšehetso tse tiileng, li thusa ho tšoara le ho hloekisa limolek'hule tse lebisitsoeng. Mokhoa ona o tsoetseng pele o nolofalelitse bo-rasaense ho fumana tlhahlobo e nepahetseng le e ka tšeptjoang, e leng se lebisang liphellong tse molemo tsa mokuli.
Ho Hloekisa Liprotheine le Ho Hlopha Lisele:
Lifaha tsa Magnetic le tsona li fumana ts'ebeliso e pharaletseng ea tlhoekiso ea liprotheine, ts'ebetso e mahlonoko ho nts'etsopele ea lithethefatsi le lipatlisiso tsa biochemistry. Ka ho kopanya li-ligands ho lifaha, bafuputsi ba ka khetha le ho ntša liprotheine tse lebisitsoeng ka lihlahisoa tse phahameng le lihlahisoa. Mokhoa ona oa tlhoekiso o potlakisa haholo ts'ebetso ea lipatlisiso ka kakaretso, e lumella bo-ramahlale ho sekaseka le ho ithuta liprotheine ka mokhoa o hlakileng haholoanyane.Ho hlopha lisele, e leng karolo ea bohlokoa ea mekhoa e fapaneng ea bongaka le ea lipatlisiso, ke karolo e 'ngoe e ruisang molemo haholo ka lifaha tsa makenete. Lifaha tsena, tse kopantsoeng le li-biomarker kapa li-antibodies, li thusa ho aroloa le ho aroloa ha lisele tse fapaneng. Ka ho sebelisa matla a khoheli, bo-rasaense ba khona ho hlophisa le ho arola lisele ho latela litšobotsi tsa tsona tsa 'mele le tsa tšebetso. Ho ba bonolo le ho nepahala ha mokhoa ona ho matlafalitse boiteko ba lipatlisiso ho utloisisa mekhoa e rarahaneng ea lisele, joalo ka tsoelo-pele ea mofetše le karabelo ea 'mele ea ho itšireletsa mafung.
Nako ea poso: Jun-25-2023